Hannah Starnes, Trainee in Project 3 with Scott Belcher, will present at poster entitled ‘Comparative Assessment of PFAS Binding of Serum Albumin across Species Using Differential Scanning Fluorimetry‘, at the 2022 Society of Toxicology (SOT) Meeting in San Diego, CA.
Per- and poly-fluorinated compounds (PFAS) are a diverse class of over eight thousand different synthetic chemicals that can be toxic and bioaccumulate in humans, livestock, and wildlife. Key toxicokinetic-related data, including PFAS-protein binding affinity values, are limited to a subset of PFAS congeners. To address this lack of binding affinity data, we developed a high-throughput in vitro differential scanning fluorimetry (DSF) assay for determining relative binding affinities of human serum albumin for individual PFAS from multiple chemical classes. We have expanded the use of this assay to test the hypothesis that there are important species specific differences in albumin binding of PFAS. To address this hypothesis we have evaluated PFAS binding of purified serum albumin from experimental and livestock species. Here we report results demonstrating the utility of this DSF assay to compare binding affinity of human, rat, rabbit, porcine and bovine serum albumins for a representative library of PFAS chemical structures. We have identified differences in binding affinity and modes of binding for individual compounds across these species. Our findings of significant differences in albumin binding across species will strengthen the relevance and applicability of non-animal computational modeling of PFAS fate, transport and distribution and increase confidence in validity of extrapolations of laboratory toxicity testing to human toxicity.
You can see Hannah’s poster presentation on Monday, March 28, 2022, 9:00am-10:45am (Pacific), Poster Session – PFAS 1, Poster Board – P188.